Summary
Macrophages, tissue-based phagocytic cells derived from blood monocytes, play important roles in immunity and homeostasis. As professional scavengers, macrophages phagocytose microbes, apoptotic and necrotic cells and take up modified lipoprotein particles. However, many of their complex interactions with other immune cells and/or various ligands are not yet clearly understood. To identify and isolate macrophage cell surface molecules, particularly macrophage receptors, for which ligands are known, a powerful generalized screening method has been established. As discussed in this chapter, this technique based on function has been successfully applied for the identification of dectin-1, the major macrophage receptor involved in the binding and recognition of β-glucans (Nature 413:36–37, 2001).
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Notes
- 1.
Glucose polymers found in the cell walls of plants, fungi and bacteria.
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Acknowledgements
This work was supported by funding from the Deutsche Forschungsgemeinschaft (DFG) to G.S. (grant SCHA 1470/1-1) and the Wellcome Trust to G.D.B.
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Schäfer, G., Brown*, G. (2009). Generation of Retroviral Macrophage cDNA Expression Libraries and Functional Screening for Surface Receptors. In: Reiner, N. (eds) Macrophages and Dendritic Cells. Methods in Molecular Biology™, vol 531. Humana Press. https://doi.org/10.1007/978-1-59745-396-7_1
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DOI: https://doi.org/10.1007/978-1-59745-396-7_1
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