Abstract
The main goal of enzyme immobilization is the industrial re-use of enzymes for many reaction cycles. In this way, simplicity and improvement of enzyme properties have to be strongly associated with the design of protocols for enzyme immobilization. In spite of their excellent catalytic properties, enzymes have many other characteristics that are not very suitable for their use in chemical industries: low stability, inhibition by high concentrations of substrates and products, low activity and selectivity toward nonnatural substrates under nonconventional conditions, and so on. The possibility of improving these unsuitable characteristics via the design of simple immobilization protocols is a very exciting goal. There are many protocols for immobilization of enzymes but very few are also very simple and/or very capable of improving enzyme properties. Novel immobilization protocols are still needed in order to achieve a massive implementation of enzymes as catalysts of the most complex chemical processes under the most benign experimental and environmental conditions. A critical review of enzyme immobilization under this point of view is still necessary.
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Guisan, J.M. (2006). Immobilization of Enzymes as the 21st Century Begins. In: Guisan, J.M. (eds) Immobilization of Enzymes and Cells. Methods in Biotechnology™, vol 22. Humana Press. https://doi.org/10.1007/978-1-59745-053-9_1
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DOI: https://doi.org/10.1007/978-1-59745-053-9_1
Publisher Name: Humana Press
Print ISBN: 978-1-58829-290-2
Online ISBN: 978-1-59745-053-9
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