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Measuring Synthesis and Degradation of MHC Class I Molecules

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Antigen Processing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1988))

Abstract

Major histocompatibility complex (MHC) class I molecules function to present pathogen derived peptides to cytotoxic T cells and act as ligands for Natural Killer cells, thus alerting the immune system to the presence of invading pathogens. However, some MHC class I molecules, most notably HLA-B27, can be strongly associated with autoimmune diseases. In addition, the MHC class I pathway is a target for numerous viral evasion strategies Understanding not only the antigen presenting functions, but also the biosynthesis and the degradation pathways of MHC class I molecules has therefore become important in determining their role in pathogen and autoimmune related diseases. Here, we describe how using epitope tagged MHC class I molecules can aid in the analysis of MHC class I molecule biosynthesis and degradation as well as complementary studies using conventional conformationally specific antibodies. Coupled together with pharmacological manipulation which can target both biosynthetic and degradative pathways, this offers a powerful tool in analyzing MHC class I molecules.

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Acknowledgments

The work was in part supported by Arthritis Research UK (grant 21261; to A.N.A. and S.J.P.).

Author information

Authors and Affiliations

  1. School of Medicine, University of St Andrews, Fife, UK

    Simon J. Powis

  2. Department of Applied Sciences, Faculty of Life and Health Sciences, Northumbria University, Newcastle upon Tyne, UK

    Antony N. Antoniou

Authors
  1. Simon J. Powis
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  2. Antony N. Antoniou
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Corresponding authors

Correspondence to Simon J. Powis or Antony N. Antoniou .

Editor information

Editors and Affiliations

  1. INSERM U1151, Université Paris Descartes, Hopital Necker-Enfants Malades, Paris, France

    Peter van Endert

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Powis, S.J., Antoniou, A.N. (2019). Measuring Synthesis and Degradation of MHC Class I Molecules. In: van Endert, P. (eds) Antigen Processing. Methods in Molecular Biology, vol 1988. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9450-2_7

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  • DOI: https://doi.org/10.1007/978-1-4939-9450-2_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9449-6

  • Online ISBN: 978-1-4939-9450-2

  • eBook Packages: Springer Protocols

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