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Soluble Regulatory Proteins for Activation of NOX Family NADPH Oxidases

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NADPH Oxidases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1982))

Abstract

NOX family NADPH oxidases deliberately produce reactive oxygen species and thus contribute to a variety of biological functions. Of seven members in the human family, the three oxidases NOX2, NOX1, and NOX3 form a heterodimer with p22phox and are regulated by soluble regulatory proteins: p47phox, its related organizer NOXO1; p67phox, its related activator NOXA1; p40phox; and the small GTPase Rac. Activation of the phagocyte oxidase NOX2 requires p47phox, p67phox, and GTP-bound Rac. In addition to these regulators, p40phox plays a crucial role when NOX2 is activated during phagocytosis. On the other hand, NOX1 activation prefers NOXO1 and NOXA1, although Rac is also involved. NOX3 constitutively produces superoxide, which is enhanced by regulatory proteins such as p47phox, NOXO1, and p67phox. Here we describe mechanisms for NOX activation with special attention to the soluble regulatory proteins.

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Acknowledgments

This work was supported in part by JSPS (Japan Society for the Promotion of Science: a Grant-in-Aid for Scientific Research on Innovative Areas “Oxygen Biology: a new criterion for integrated understanding of life” No. 26111009).

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Authors and Affiliations

  1. Department of Biochemistry, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan

    Hideki Sumimoto, Reiko Minakami & Kei Miyano

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  1. Hideki Sumimoto
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  2. Reiko Minakami
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  3. Kei Miyano
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Correspondence to Hideki Sumimoto .

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Editors and Affiliations

  1. Conway Institute, School of Medicine, University College Dublin, Dublin, Ireland

    Ulla G. Knaus

  2. Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA

    Thomas L. Leto

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Sumimoto, H., Minakami, R., Miyano, K. (2019). Soluble Regulatory Proteins for Activation of NOX Family NADPH Oxidases. In: Knaus, U., Leto, T. (eds) NADPH Oxidases. Methods in Molecular Biology, vol 1982. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9424-3_8

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  • DOI: https://doi.org/10.1007/978-1-4939-9424-3_8

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