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Preparation of a Dithiol-Reactive Probe for PET Imaging of Cell Death

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Functional Disulphide Bonds

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1967))

Abstract

Conjugates of 4-(N-(S-glutathionylacetyl)amino)phenylarsonous acid (GSAO) with optical or radionuclide probes are able to image cell death in vivo. GSAO conjugates are retained in the cytosol of dying and dead cells via the formation of covalent bonds between the As(III) ion and the thiol groups of proximal cysteine residues. Here we describe the method for preparing a NODAGA-GSAO conjugate and its radiolabeling with gallium-68 (68Ga-NODAGA-GSAO) for positron-emission tomography (PET) imaging of cell death.

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Author information

Authors and Affiliations

  1. Department of Nuclear Medicine and PET, Prince of Wales Hospital, Randwick, NSW, Australia

    Ivan Ho Shon & Jennifer Guille

  2. Prince of Wales Clinical School, University of New South Wales, Sydney, NSW, Australia

    Ivan Ho Shon

  3. The Centenary Institute, NHMRC Clinical Trials Centre, Sydney Medical School, The University of Sydney, Sydney, NSW, Australia

    Ivan Ho Shon & Philip Hogg

  4. School of Medical Sciences (Pharmacology), Sydney Medical School, The University of Sydney, Sydney, NSW, Australia

    Michael P. Gotsbacher & Rachel Codd

  5. Department of Nuclear Medicine and PET, Fiona Stanley Hospital, Murdoch, WA, Australia

    Divesh Kumar

Authors
  1. Ivan Ho Shon
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  2. Michael P. Gotsbacher
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  3. Jennifer Guille
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  4. Divesh Kumar
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  5. Rachel Codd
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  6. Philip Hogg
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Corresponding author

Correspondence to Ivan Ho Shon .

Editor information

Editors and Affiliations

  1. The Centenary Institute, NHMRC Clinical Trials Centre, Sydney Medical School, University of Sydney, Sydney, NSW, Australia

    Philip Hogg

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Ho Shon, I., Gotsbacher, M.P., Guille, J., Kumar, D., Codd, R., Hogg, P. (2019). Preparation of a Dithiol-Reactive Probe for PET Imaging of Cell Death. In: Hogg, P. (eds) Functional Disulphide Bonds. Methods in Molecular Biology, vol 1967. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9187-7_19

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  • DOI: https://doi.org/10.1007/978-1-4939-9187-7_19

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9186-0

  • Online ISBN: 978-1-4939-9187-7

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