Abstract
Here we summarize how glycosphingolipid production can be followed using metabolic labeling with radiolabeled lipid precursors. No assays are available yet that directly would address the lipid transfer protein activity in vivo. Therefore, these approaches can serve as tools to indirectly study the lipid transfer protein activity in cells, by monitoring their impact on the glycosphingolipid homeostasis.
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Acknowledgments
This work was supported by Svenska Kulturfonden, Sigrid Jusélius Foundation, Magnus Ehrnrooth Foundation, Medicinska Understödsföreningen Liv och Hälsa, Svensk Österbottniska Samfundet.
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Backman, A.P.E., Halin, J., Kjellberg, M.A., Mattjus, P. (2019). Indirect Lipid Transfer Protein Activity Measurements Using Quantification of Glycosphingolipid Production. In: Drin, G. (eds) Intracellular Lipid Transport. Methods in Molecular Biology, vol 1949. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9136-5_9
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DOI: https://doi.org/10.1007/978-1-4939-9136-5_9
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