Abstract
The majority of proteins in eukaryotic cells are subject to amino-terminal (Nt) acetylation. Recombinant protein expressed using prokaryotic expression systems such as E. coli would not normally be Nt-acetylated as these cells lack the appropriate N-α-terminal acetylation complex. Here we describe a simple protocol that allows the recombinant expression and purification of Nt-acetylated alpha-synuclein (aS) from E. coli.
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References
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Acknowledgments
Work in this lab is supported by the University of Kent, BBSRC, and the Royal Society.
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Eastwood, T.A., Mulvihill, D.P. (2019). Recombinant Expression and Purification of N-Acetylated Alpha-Synuclein. In: Bartels, T. (eds) Alpha-Synuclein. Methods in Molecular Biology, vol 1948. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9124-2_10
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DOI: https://doi.org/10.1007/978-1-4939-9124-2_10
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