Abstract
Next-generation sequencing (NGS) is rapidly becoming the method of choice for mutation analysis in both research and diagnostics. The benefit of targeted NGS compared to whole-genome and whole-exome sequencing is that smaller amounts of input material can be used as well as qualitatively suboptimal tissue samples, like formalin-fixed, paraffin-embedded archival tissue.
Here, we describe the protocol for targeted next-generation sequencing using the Ion Torrent PGM platform in combination with Ion Ampliseq NGS gene panels for formalin-fixed, paraffin-embedded tissues. Both the manual and the automated workflow are described as well as the bioinformatics for data analysis.
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Strengman, E., Barendrecht-Smouter, F.A.S., de Voijs, C., de Vree, P., Nijman, I.J., de Leng, W.W.J. (2019). Amplicon-Based Targeted Next-Generation Sequencing of Formalin-Fixed, Paraffin-Embedded Tissue. In: Murray, S. (eds) Tumor Profiling. Methods in Molecular Biology, vol 1908. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9004-7_1
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DOI: https://doi.org/10.1007/978-1-4939-9004-7_1
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-9002-3
Online ISBN: 978-1-4939-9004-7
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