Abstract
Clinically apparent tumors have often established an immunosuppressive tumor microenvironment which renders them “cold,” meaning that there are low numbers of immune cells within the tumor. Consequently, novel immunotherapy approaches such as checkpoint inhibitors fail to reactivate the tumor-targeted immune cells. Here we describe the generation of heterotypic tumor-stroma spheroids to study various approaches aiming at the reactivation of cancer immunosurveillance. These spheroids allow to investigate whether a certain immunotherapy or a combination treatment is able to stimulate antitumor immunity in poorly immunological (“cold”) tumors, by increasing the number of tumor-infiltrating immune cells (“hot” tumors).
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References
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The technical support of Lisa Bauer is highly acknowledged.
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Zboralski, D., Frömming, A. (2019). Generation and Harnessing of Heterotypic Tumor-Stroma Spheroids to Study Cancer Immunosurveillance. In: López-Soto, A., Folgueras, A. (eds) Cancer Immunosurveillance. Methods in Molecular Biology, vol 1884. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8885-3_19
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DOI: https://doi.org/10.1007/978-1-4939-8885-3_19
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8884-6
Online ISBN: 978-1-4939-8885-3
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