Abstract
Despite many advancements in brain cancer therapeutics, brain cancer remains one of the most elusive diseases with high migratory capacity and a dismal prognosis. It is well established that tumor stem cells utilize the same available migratory machinery that normal cells employ. Some of the major determinants of brain tumor stem cell migration are their cytoskeletal rearrangements and adhesion dynamics. This phenomenon allows brain tumor stem cells to perpetually migrate, invade, and repopulate in a vicious cycle leading to tumor expansion and invasion at tumor boundaries. In order to dissect the enabling factors that allow for this process to be hijacked, we have identified relevant assays to enable measurements of neoplastic migration such as Boyden Chamber, 3D chemogradient chamber, Nanopattern, and wound healing assays. Our purpose is to report the complex experimental platforms seen in the literature today and provide an optimal platform to kick off your studies in this field.
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References
Smith CL, Kilic O, Schiapparelli P, Guerrero-Cazares H, Kim DH, Sedora-Roman NI et al (2016) Migration phenotype of brain-Cancer cells predicts patient outcomes. Cell Rep 15(12):2616–2624
van Zijl F, Krupitza G, Mikulits W (2011) Initial steps of metastasis: cell invasion and endothelial transmigration. Mutat Res 728(1–2):23–34
Friedl P, Wolf K (2010) Plasticity of cell migration: a multiscale tuning model. J Cell Biol 188(1):11–19
Petrie RJ, Yamada KM (2012) At the leading edge of three-dimensional cell migration. J Cell Sci 125(Pt 24):5917–5926
Nina Kramera AW, Ungera C, Rosnera M, Krupitzab G, Hengstschlägera M, Dolznig H (2012) In vitro cell migration and invasion assays. Mutat Res 752:10–24 ElSevier
Tilghman J, Schiapparelli P, Lal B, Ying M, Quinones-Hinojosa A, Xia S et al (2016) Regulation of Glioblastoma tumor-propagating cells by the integrin partner Tetraspanin CD151. Neoplasia 18(3):185–198
Acknowledgments
AQH was supported by the Mayo Clinic Professorship and a Clinician Investigator award as well as the NIH (R43CA221490, R01CA200399, R01CA183827, R01CA195503, R01CA216855). MLV was supported by CONACYT and PECEM from the National Autonomous University of Mexico. We would like to thank Hugo Guerrero-Cazares for his contribution to figures in this chapter.
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Lara-Velazquez, M., Al-kharboosh, R., Prieto, L., Schiapparelli, P., Quiñones-Hinojosa, A. (2019). The Study of Brain Tumor Stem Cell Migration. In: Singh, S., Venugopal, C. (eds) Brain Tumor Stem Cells. Methods in Molecular Biology, vol 1869. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8805-1_9
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DOI: https://doi.org/10.1007/978-1-4939-8805-1_9
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