Abstract
Here, we offer you a high-throughput assay to measure the ATP synthesis capacity in cells or isolated mitochondria. More specifically, the assay is linked to the mitochondrial’ electron transport chain components of your interest being either through complex I (with or without a linkage to pyruvate dehydrogenase activity), through complex II, or through the electron transport flavoprotein and complex I (β-oxidation of fatty acids).
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Acknowledgments
DC and PV are senior clinical investigators of the Research Foundation-Flanders (FWO-Belgium).
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The authors declare no competing interests.
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Bird, M.J., Radenkovic, S., Vermeersch, P., Cassiman, D. (2019). Measuring Rates of ATP Synthesis. In: Fendt, SM., Lunt, S. (eds) Metabolic Signaling. Methods in Molecular Biology, vol 1862. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8769-6_7
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DOI: https://doi.org/10.1007/978-1-4939-8769-6_7
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8768-9
Online ISBN: 978-1-4939-8769-6
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