Abstract
The tumor microenvironment is a complex network of interacting cells composed of immune and nonimmune cells. It has been reported that the composition of the immune contexture has a significant impact on tumor growth and patient survival in different solid tumors. For instance, we and other groups have previously demonstrated that a strong infiltration of T-helper type 1 (Th1) or memory CD8+ T cells is associated with long-term survival of cancer patients. Nevertheless, the prognostic value of the other immune populations, namely regulatory T cells (Treg), B cells, and gamma delta (γδ) T cells, remains a matter of debate. Herein, we describe novel flow cytometry-based strategies to sort out these different immune populations in order to evaluate their role in non-small cell lung cancer (NSCLC).
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Acknowledgments
We thank all present and past members of the Laboratory “Cancer, Immune control and Escape” (UMRS1138 INSERM, Cordeliers Research Center), pathologists, and clinicians who have participated in these studies, for their help and valuable discussions. We are grateful to lung cancer patients involved in this research performed in our laboratory.
Funding: This work was supported by the “Institut National de la Santé et de la Recherche Médicale (INSERM), Pierre and Marie Curie University, Paris-Descartes University, the Labex Immuno-Oncology (LAXE62_9UMRS872 Fridman), CARPEM (Cancer Research for PErsonalized Medicine), Institut National du Cancer (INCa-PLBio 2014-2017 GDSTRESS), and Fondation ARC pour la Recherche sur le Cancer. Priyanka Devi-Marulkar was supported by a grant from the Fondation ARC pour la Recherche sur le Cancer. Hélène Kaplon was supported by a grant from the Ligue Nationale contre le Cancer.
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Devi-Marulkar, P., Kaplon, H., Dieu-Nosjean, MC., Lawand, M. (2018). Designed Methods for the Sorting of Tertiary Lymphoid Structure-Immune Cell Populations. In: Dieu-Nosjean, MC. (eds) Tertiary Lymphoid Structures. Methods in Molecular Biology, vol 1845. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8709-2_11
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DOI: https://doi.org/10.1007/978-1-4939-8709-2_11
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