Abstract
The research and application of regeneration medicine will require a reliable source of stem cells. Adipose tissue has proven to be an easily accessible and rich source of adult stem cells, termed adipose-derived stem cells (ASCs). ASCs have the most important advantage over stem cells from other available sources. There is no other human tissue as abundant as adipose tissue, making it possible to isolate adequate numbers of ASCs for potential clinical applications. Here, we describe detailed methods for isolating and characterizing ASCs. These procedures can be applied to adipose tissue not only for humans but also for other species.
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References
Zuk PA (2010) The adipose-derived stem cell: looking back and looking ahead. Mol Biol Cell 21(11):1783–1787
Gimble JM, Nuttall ME (2011) Adipose-derived stromal/stem cells (ASC) in regenerative medicine: pharmaceutical applications. Curr Pharm Des 17(4):332–339
Gimble JM, Bunnell BA, Guilak F (2012) Human adipose-derived cells: an update on the transition to clinical translation. Regen Med 7(2):225–235
Santos FD, Andrade PZ, Abecasis MM, Gimble JM, Chase LG, Campbell AM, Boucher S, Vemuri MC, Silva CL, Cabral JM (2011) Toward a clinical-grade expansion of mesenchymal stem cells from human sources: a microcarrier-based culture system under xeno-free conditions. Tissue Eng Part C Methods 17(12):1201–1210
Mizuno H, Tobita M, Uysal AC (2012) Adipose-derived stem cells as a novel tool for future regenerative medicine. Stem Cells 30(5):804–810
Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Dj P, Horwitz E (2006) Cytotherapy 8(4):315–317
Bunnell BA, Flaat M, Gagliardi C, Patel B, Ripoll C (2008) Adipose-derived stem cells: isolation, expansion and differentiation. Methods 45(2):115–120
Kretlow JD, Jin YQ, Liu W, Zhang WJ, Hong TH, Zhou G, Baggett LS, Mikos AG, Cao Y (2008) Donor age and cell passage affects differentiation potential of murine bone marrow-derived stem cells. BMC Cell Biol 9:60
Acknowledgments
This work was financially supported by the Natural Science Foundation of China (Research number 31271027 and 81171475).
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Liu, G., Chen, X. (2018). Isolating and Characterizing Adipose-Derived Stem Cells. In: Singh, S., Rameshwar, P. (eds) Somatic Stem Cells. Methods in Molecular Biology, vol 1842. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8697-2_13
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DOI: https://doi.org/10.1007/978-1-4939-8697-2_13
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Publisher Name: Humana Press, New York, NY
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