Abstract
In vitro generated mammalian cardiomyocytes provide experimental models for studying normal mammalian cardiomyocyte development, for disease modeling and for drug development. They also promise to inform future therapeutic strategies for repair of injured or diseased myocardium. Here we provide reliable protocols for differentiation of mouse embryonic stem cells into functional cardiomyocytes, together with Notes about trouble shooting and optimizing such protocols for specific cell lines.
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Acknowledgments
We thank the Bruneau lab (Gladstone Institute) and the Martinez-Arias lab (University of Cambridge) for helpful advice regarding mouse ES cell culture. We thank Kate Watt and Yvonne Turnbull (University of Aberdeen), for lab management and technical support, the British Heart Foundation (PG/13/23/30080) for research funding, and the Institute of Medical Sciences (University of Aberdeen) for PhD studentship funding.
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Lynch, A.T., Mazzotta, S., Hoppler, S. (2018). Cardiomyocyte Differentiation from Mouse Embryonic Stem Cells. In: Ishikawa, K. (eds) Experimental Models of Cardiovascular Diseases. Methods in Molecular Biology, vol 1816. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8597-5_4
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DOI: https://doi.org/10.1007/978-1-4939-8597-5_4
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