Abstract
Increase in cardiac afterload as represented by hypertension is an established risk factor for cardiovascular diseases. Animal models of increased cardiac afterload offer studies aiming at identifying key molecular mechanisms and developing new therapeutic approaches. We have reported that banding of the ascending aorta in pigs results in significant cardiac hypertrophy and increased myocardial fibrosis at the chronic stages. These changes were accompanied by increased stiffness of the heart, but not by systolic dysfunction. In this chapter, we describe methods to surgically band the ascending aorta in pigs. After 3 months, animals develop systolic left ventricular pressure of >200 mmHg with above described changes in the heart.
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Acknowledgments
This work is supported by NIH R01 HL139963 (K.I.) and AHA-SDG 17SDG33410873 (K.I.). O.B. was supported by the Deutsche Herzstiftung.
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Bikou, O., Miyashita, S., Ishikawa, K. (2018). Pig Model of Increased Cardiac Afterload Induced by Ascending Aortic Banding. In: Ishikawa, K. (eds) Experimental Models of Cardiovascular Diseases. Methods in Molecular Biology, vol 1816. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8597-5_26
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DOI: https://doi.org/10.1007/978-1-4939-8597-5_26
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