Abstract
Temporary or permanent left coronary artery (LCA) ligation is the most widely used model of heart failure. In the present protocol, we describe the materials necessary for the procedure, key steps of the LCA ligation, triphenyl tetrazolium chloride (TTC) staining, and calculation of myocardial infarction (MI) size after ischemia–reperfusion (I/R) injury (30 min/24 h) in rats and mice. We discuss precautions and tips regarding the operation before and after surgery, both in vivo and ex vivo. The aim of this chapter is to describe the details of LCA surgery and provide recommendations for current and future surgical operators.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Houser SR, Margulies KB, Murphy AM, Spinale FG, Francis GS, Prabhu SD, Rockman HA, Kass DA, Molkentin JD, Sussman MA, Koch WJ, American Heart Association Council on Basic Cardiovascular Sciences, Council on Clinical Cardiology, Council on Functional Genomics and Translational Biology (2012) Animal models of heart failure: a scientific statement from the American Heart Association. Circ Res 111(1):131–150. https://doi.org/10.1161/RES.0b013e3182582523
Patten RD, Hall-Porter MR (2009) Small animal models of heart failure: development of novel therapies, past and present. Circ Heart Fail 2(2):138–144. https://doi.org/10.1161/CIRCHEARTFAILURE.108.839761
Chen J, Ceholski DK, Liang L, Fish K, Hajjar RJ (2017) Variability in coronary artery anatomy affects consistency of cardiac damage following myocardial infarction in mice. Am J Physiol Heart Circ Physiol 313(2):H275–H282. https://doi.org/10.1152/ajpheart.00127.2017
Chen J, Hammoudi N, Benard L, Ceholski DK, Zhang S, Lebeche D, Hajjar RJ (2016) The probability of inconstancy in assessment of cardiac function post-myocardial infarction in mice. Cardiovasc Pharm Open Access 5(5). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5130155/
Chen J, Chemaly ER, Liang LF, LaRocca TJ, Yaniz-Galende E, Hajjar RJ (2011) A new model of congestive heart failure in rats. Am J Phys Heart Circ Phys 301(3):H994–H1003. https://doi.org/10.1152/ajpheart.00245.2011
Chen J, Chemaly E, Liang L, Kho C, Lee A, Park J, Altman P, Schecter AD, Hajjar RJ, Tarzami ST (2010) Effects of CXCR4 gene transfer on cardiac function after ischemia-reperfusion injury. Am J Pathol 176(4):1705–1715. https://doi.org/10.2353/ajpath.2010.090451
Acknowledgments
This work is supported by NIH R01 HL117505, HL 119046, HL129814, 128072, HL131404, R01HL135093, a P50 HL112324, and two Transatlantic Fondation Leducq grants. We would like to acknowledge the Gene Therapy Resource Program (GTRP) of the National Heart, Lung, and Blood Institute, National Institutes of Health. DKC is supported by a fellowship from the American Heart Association (15POST25090116). ICT is supported by NIH/NHLBI K01 HL 133424-01.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Chen, J., Ceholski, D.K., Turnbull, I.C., Liang, L., Hajjar, R.J. (2018). Ischemic Model of Heart Failure in Rats and Mice. In: Ishikawa, K. (eds) Experimental Models of Cardiovascular Diseases. Methods in Molecular Biology, vol 1816. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8597-5_13
Download citation
DOI: https://doi.org/10.1007/978-1-4939-8597-5_13
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8596-8
Online ISBN: 978-1-4939-8597-5
eBook Packages: Springer Protocols