Abstract
Posttranscriptional regulation of RNA is an important component of gene expression by controlling the total amount of mRNA available for translation into protein. It involves multiple pathways including nuclear processing of mRNA and its precursors, RNA silencing, and regulation of RNA decay. Poly(ADP-ribose) polymerases (PARPs), enzymes that modify target proteins with ADP-ribose, play important roles in several RNA-regulatory pathways. RNA-binding PARPs target specific transcripts for regulation, and multiple PARPs ADP-ribosylate RNA-regulatory proteins to alter their localization, activity, or RNA binding. Additionally, RNA-binding proteins can bind directly to poly(ADP-ribose) with various effects on their function. Here we describe methods to identify and confirm specific transcripts that are regulated by PARPs.
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Acknowledgments
This work was partially supported by Cancer Center Support (core; grant P30-CA14051) and RO1GM087465 from the National Institutes of Health to PC. FJB was funded by a Ludwig Postdoctoral Fellowship.
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Bock, F.J., Chang, P. (2018). Identifying Target RNAs of PARPs. In: Chang, P. (eds) ADP-ribosylation and NAD+ Utilizing Enzymes. Methods in Molecular Biology, vol 1813. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-8588-3_23
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DOI: https://doi.org/10.1007/978-1-4939-8588-3_23
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