Abstract
The timely recruitment of innate and adaptive immune cells to sites of inflammation and repair is essential for host defense against pathogens and repair of damaged tissues. The development of bioassays such as in vitro chemotaxis assays played an important role in the original purification of chemoattractant cytokines including interleukin-1 and the CC and CXC chemokines. The earliest chemotaxis methods were based on the principle of the Boyden chamber, first described in 1962. In this chapter we give detailed protocols for more recent techniques that allow determination of macrophage chemotaxis in real time. These techniques have given new insights into the regulation of macrophage responses to chemotaxis in vitro and in vivo.
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Acknowledgments
Asif Iqbal is supported by a Birmingham Fellowship and acknowledges support from the BHF Centre of Research Excellence, Oxford (RE/13/1/30181). Work in the Greaves laboratory is funded by the British Heart Foundation (RG/15/10/31485), the EP Abraham Research Fund (EP 238), and the Nova Nordisk Fund (652.NNF15CC0018346).
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Taylor, L., Recio, C., Greaves, D.R., Iqbal, A.J. (2018). In Vitro Migration Assays. In: Rousselet, G. (eds) Macrophages. Methods in Molecular Biology, vol 1784. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7837-3_19
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DOI: https://doi.org/10.1007/978-1-4939-7837-3_19
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