Abstract
Nature has designed nanosized particles, specifically viruses, equipped to deliver cargo to cells. We report the chemical bioconjugation and shape shifting of a hollow, rod-shaped tobacco mosaic virus (TMV) to dense spherical nanoparticles (SNPs). We describe methods to transform TMV rods to spheres, load TMV rods and spheres with the chemotherapeutic drug, doxorubicin (DOX), to deliver modified particles to breast cancer cells, and to determine the IC50 values of the plant virus-based drug delivery system.
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Acknowledgments
This work was supported in part by a grant from the National Science Foundation (DMR 1452257 to N.F.S.).
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Bruckman, M.A., Czapar, A.E., Steinmetz, N.F. (2018). Drug-Loaded Plant-Virus Based Nanoparticles for Cancer Drug Delivery. In: Wege, C., Lomonossoff, G. (eds) Virus-Derived Nanoparticles for Advanced Technologies. Methods in Molecular Biology, vol 1776. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7808-3_28
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DOI: https://doi.org/10.1007/978-1-4939-7808-3_28
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