Abstract
The generation of virus-free plants is a key requirement in the international trade of orchid seedlings. Shoot apical meristem isolation and its successful culture is one of the methods that can generate virus-free plants. In this chapter, a general protocol for meristem isolation and subsequent micropropagation of meristem explants and regenerated plantlets for Phalaenopsis is detailed. Patience, practice, and cleanliness are key elements in shoot apical meristem excision. Information on the temporary immersion system in the mass propagation of virus-free Phalaenopsis plantlets is also presented.
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Shen, RS., Hsu, ST. (2018). Virus Elimination Through Meristem Culture and Rapid Clonal Propagation Using a Temporary Immersion System. In: Lee, YI., Yeung, ET. (eds) Orchid Propagation: From Laboratories to Greenhouses—Methods and Protocols. Springer Protocols Handbooks. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7771-0_14
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DOI: https://doi.org/10.1007/978-1-4939-7771-0_14
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