Abstract
Plant postembryonic development takes place in region called meristems that represent a reserve of undifferentiated cells. In the root meristem of Arabidopsis thaliana, all tissues originate from a stem-cell niche. Stem-cell daughters undergo a finite number of cell divisions until they reach the transition zone where divisions cease and cells start to differentiate. For meristem maintenance, and therefore continuous root growth, the rate of cell differentiation must equal the rate of generation of new cells. How this balance is achieved is a central question in plant biology. In this chapter we described protocols to help the operator in approaching developmental studies on the Arabidopsis root meristem.
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References
Scheres B (2007) Stem-cell niches: nursery rhymes across kingdoms. Nat Rev Mol Cell Biol 8:345–354
van den Berg C, Willemsen V, Hendriks G et al (1997) Short-range control of cell differentiation in the Arabidopsis root meristem. Nature 390:287–289
Rougier M (1981) Secretory activity of the root cap. In: Tanner W, Loewus FA (eds) Plant carbohydrates II. PLANT, vol 13B. Springer, Berlin, Heidelberg, pp 542–574
Sievers A, Hensel W (1991) Root cap. In: Waisel Y, Eshel A, Kafkfi U (eds) Plant roots: the hidden half. Marcel Dekker, New York, pp 53–74
Dolan L, Janmaat K, Willemsen V et al (1993) Cellular organisation of the Arabidopsis thaliana root. Development 119:71–84
Berger F, Hung CY, Dolan L et al (1998) Control of cell division in the root epidermis of Arabidopsis thaliana. Dev Biol 194:235–245
Galway ME, Masucci JD, Lloyd AM et al (1994) The TTG gene is required to specify epidermal cell fate and cell patterning in the Arabidopsis root. Dev Biol 166:740–754
Helariutta Y, Fukaki H, Wysocka-Diller J et al (2000) The SHORT-ROOT gene controls radial patterning of the Arabidopsis root through radial signaling. Cell 101:505–517
Leyser O, Day D (2005) Mechanism in plant development. Blackwell Publishing, Malden, USA
De Smet I, Vanneste S, Inzè D et al (2006) Lateral root initiation or the birth of a new meristem. Plant Mol Biol 60:871–887
Benfey PN, Scheres B (2000) Root development. Curr Biol 10:R813–R815
Di Mambro R, De Ruvo M, Pacifici E, Salvi E, Sozzani R, Benfey PN, Busch W, Novak O, Ljung K, Di Paola L, Marée AFM, Costantino P, Grieneisen VA, Sabatini S (2017) Auxin minimum triggers the developmental switch from cell division to cell differentiation in the root. Proc Natl Acad Sci U S A 114(36):E7641–E7649
Dello Ioio R, Linhares FS, Scacchi E et al (2007) Cytokinins determine Arabidopsis root-meristem size by controlling cell differentiation. Curr Biol 17:678–682
Beemster GTS, Baskin TI (1998) Analysis of cell division and elongation underlying the developmental acceleration of root growth in Arabidopsis thaliana. Plant Physiol 116:1515–1526
Scheres B, Wolkenfelt H, Willemsen V et al (1994) Embryonic origin of the Arabidopsis primary root and root meristem initials. Development 120:2475–2487
Moubayidin L, Perilli S, Dello Ioio R et al (2010) The rate of cell differentiation controls the Arabidopsis root meristem growth phase. Curr Biol 20:1138–1143
Dello Ioio R, Nakamura K, Moubayidin L et al (2008) A genetic framework for the control of cell division and differentiation in the root meristem. Science 322:1380–1384
Mayer U, Torres Ruis R, Berleth T et al (1991) Mutations affecting body organization in the Arabidopsis embryo. Nature 353:402–407
French AP, Wilson MH, Kenobi K et al (2012) Identifying biological landmarks using a novel cell measuring image analysis tool: cell-o-tape. Plant Methods 8:7
Musielak TJ, Schenkel L, Kolb M, Henschen A, Bayer M (2015) A simple and versatile cell wall staining protocol to study plant reproduction. Plant Reproduction 28(3-4):161–169
Acknowledgment
The authors would like to thank Raffaele Dello Ioio, Rosangela Sozzani, Elena Pacifici, Laura Polverari, Emanuela Pierdonati, Elena Salvi, and Serena Perilli for helpful comment. This work was supported by The European Research Council.
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Di Mambro, R., Sabatini, S. (2018). Developmental Analysis of Arabidopsis Root Meristem. In: Ristova, D., Barbez, E. (eds) Root Development. Methods in Molecular Biology, vol 1761. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7747-5_3
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DOI: https://doi.org/10.1007/978-1-4939-7747-5_3
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