Abstract
The processes by which the intra-abdominal organ circulatory system develops in the embryo and during organogenesis are unclear. Previous studies have used fixed tissues to study the development of abdominal organ vasculature in the embryo; however, the intravital circulation of intra-abdominal organs in rodent fetal development has not been studied. This protocol describes a system that uses two-photon laser-scanning microscopy (TPLSM) for real-time observation and quantification of normal and pathologic live fetal intra-abdominal dynamics while the fetus is still connected to the mother via the umbilical cord.
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Koike, Y. et al. (2018). Live Imaging of Fetal Intra-abdominal Organs Using Two-Photon Laser-Scanning Microscopy. In: Delgado-Olguin, P. (eds) Mouse Embryogenesis. Methods in Molecular Biology, vol 1752. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7714-7_6
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DOI: https://doi.org/10.1007/978-1-4939-7714-7_6
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7713-0
Online ISBN: 978-1-4939-7714-7
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