Abstract
Carbon (C) and nitrogen (N) are essential elements for metabolism, and the ratio of C to N availability is called the C/N balance. C/N balance is very important for plant growth, but little is known about the detailed mechanisms of plant C/N responses. Previously a method of treating Arabidopsis plants with sugar-supplemented medium for studying C/N responses at early post-germinative growth stages has been developed. This method, however, cannot be used to determine physiological C/N effects in plants of mature growth stages, including senescence. Here we present two methods of analyzing responses to C/N treatments in senescing plants: transient C/N treatment with liquid medium and long-term C/N treatment with elevated atmospheric CO2.
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Acknowledgments
We thank Dr. Juntaro Negi (Kyushu University) for technical advice on the CO2 manipulation system and Drs. Takushi Hachiya (Nagoya University) and Junpei Takano (Osaka Prefecture University) for technical advice on the hydroponic culture method. This work was supported by the Japan Society for the Promotion of Science (JSPS) Grants-in-Aid for Scientific Research (No. 24770035, 15K18819 and 17K08190) to TS, on Innovation Areas (No. 24114701 and No. 25112501) to JY, and in part by The Akiyama Foundation to TS. SA was supported by the Research Fellowship for Young Scientists from the JSPS (15J01802) and the Plant Global Education Project from the Nara Institute of Science and Technology (2013–2014).
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Aoyama, S., Yamaguchi, J., Sato, T. (2018). Methods for Elucidation of Plant Senescence in Response to C/N-Nutrient Balance. In: Guo, Y. (eds) Plant Senescence. Methods in Molecular Biology, vol 1744. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7672-0_14
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DOI: https://doi.org/10.1007/978-1-4939-7672-0_14
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Online ISBN: 978-1-4939-7672-0
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