Abstract
Lipids play a key role in the signaling pathways of cancer, cardiovascular, diabetic, and inflammatory diseases. A major challenge in the analysis of lipids is the many isobaric interferences present in highly complex samples that confound identification and accurate quantitation. After obtaining the total lipid extract from a sample, differential mobility separation has proven to be a powerful tool for gas-phase fractionation of lipid classes. When combined with mass spectrometry, this allows the unambiguous identification and thus quantification of lipid molecular species. These components, sample extraction, gas-phase separation, and mass spectrometry, form the basis of a novel integrated quantitative lipid analysis platform.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Folch J, Lees M, Stanley GHS (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37(8):911–917
Matyash V, Liebisch G, Kurzchalia TV, Shevchenko A, Schwudke D (2008) Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics. J Lipid Res 49(5):1137–1146
Lintonen TPI, Baker PRS, Suoniemi M, Ubhi BK, Koistinen KM, Duchoslav E, Campbell JL, Ekroos K (2014) Differential mobility spectrometry-driven shotgun lipidomics. Anal Chem 86(19):9662–9669
Ubhi BK, Watkins S (2015) High quality, reproducible, lipidomics: making lipid research routinely accessible. Chromatography Today
Acknowledgments
The author would like to thank the Lipidyzer development team at Metabolon (Raleigh, NC) and the Lipidyzer development team at SCIEX (Framingham, MA) including Avanti Polar Lipids (Alabaster, AL) for manufacturing the novel internal standards accompanying the Lipidyzer Platform.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Ubhi, B.K. (2018). Direct Infusion-Tandem Mass Spectrometry (DI-MS/MS) Analysis of Complex Lipids in Human Plasma and Serum Using the Lipidyzer™ Platform. In: Giera, M. (eds) Clinical Metabolomics. Methods in Molecular Biology, vol 1730. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7592-1_15
Download citation
DOI: https://doi.org/10.1007/978-1-4939-7592-1_15
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7591-4
Online ISBN: 978-1-4939-7592-1
eBook Packages: Springer Protocols