Abstract
In differential peptidomics, peptide profiles are compared between biological samples and the resulting expression levels are correlated to a phenotype of interest. This, in turn, allows us insight into how peptides may affect the phenotype of interest. In quantitative differential peptidomics, both label-based and label-free techniques are often employed. Label-based techniques have several advantages over label-free methods, primarily that labels allow for various samples to be pooled prior to liquid chromatography-mass spectrometry (LC-MS) analysis, reducing between-run variation. Here, we detail a method for performing quantitative peptidomics using stable amine-binding isotopic and isobaric tags.
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Acknowledgments
W.D.H. is a research fellow of the FWO-Flanders. The authors wish to thank the FWO (G069713 and G095915N) and KU Leuven internal funds (C14/15/049) and the European Research Council (ERC grant 586 340318) for financial support.
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Boonen, K. et al. (2018). Quantitative Peptidomics with Isotopic and Isobaric Tags. In: Schrader, M., Fricker, L. (eds) Peptidomics. Methods in Molecular Biology, vol 1719. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7537-2_9
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DOI: https://doi.org/10.1007/978-1-4939-7537-2_9
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