Abstract
Cancer stem Cells or Cancer Stem-like Cells are thought to be associated with chemoresistance and recurrence in cancer patients following chemotherapy. Developing a method to study these malignant populations is the key to successful development of drug or immunotherapeutic assays. Here, we present a method of identification, isolation of Prostate Cancer Stem Cells (PCSCs) from the DU145 prostate cancer cell line using the NANOG-GFP expression system.
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Acknowledgment
This work was supported by the John and Lucille van Geest Foundation, and the John van Geest Cancer Research Centre, Nottingham Trent University.
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Buczek, M.E., Reeder, S.P., Regad, T. (2018). Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System. In: Papaccio, G., Desiderio, V. (eds) Cancer Stem Cells. Methods in Molecular Biology, vol 1692. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7401-6_13
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DOI: https://doi.org/10.1007/978-1-4939-7401-6_13
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