Abstract
In traditional wheat breeding, the uniformity of lines derived from a breeding population is obtained by repeated selfing from the F1 which takes several generations to reach homozygosity in loci controlling traits of interest. Using doubled haploid technology, however, it is possible to attain 100% homozygosity at all loci in a single generation and completely homogeneous breeding lines can be obtained in 1–2 years. Thus, doubled haploid technology may significantly reduce cultivar development time. Two major methods for producing wheat doubled haploids are androgenesis (anther culture and microspore culture) and embryo culture using wheat–maize wide hybridization, the latter being the most effective and widely used method. The method of wide hybridization between wheat and maize is laborious but is widely successful for rapidly obtaining homozygous lines. This technique includes six major steps: emasculation of the wheat flower; pollination of the emasculated flower with maize pollen; hormone treatment; embryo rescue; haploid plant regeneration in tissue culture medium; and chromosome doubling. It has been observed that the efficiency of doubled haploid production depends on both maize and wheat genotypes, good plant health and proper greenhouse conditions (without disease, insects, or drought stress), and proper conduct of all procedures. Therefore, the procedures may need minor modification in order to produce higher numbers of embryos, haploid green plants, and doubled haploid plants.
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References
Maluszynski M, Kasha KJ, Szarejko I (2003) Published doubled haploid protocols in plant species. In: Maluszynski M et al (eds) Doubled haploid production in crop plants. Kluwer Academic Publishers, Dordrecht, pp 309–335
Forster BP, Thomas WTB (2005) Doubled haploids in genetics and plant breeding. In: Janick J (ed) Plant breeding reviews, vol 25. John Wiley & Sons, Inc., Hoboken, NJ, pp 57–88
Niu Z, Jiang A, Hammad WA et al (2014) Review of doubled haploid production in durum and common through wheat x maize hybridization. Plant Breed 133:313–320
Barclay IR (1975) High frequencies of haploid production in wheat (Triticum aestivum) by chromosome elimination. Nature 256:410–411
Laurie DA, Bennet MD (1986) Wheat x maize hybridization. Can J Genet Cytol 28:313–316
Suenaga K, Nakajima K (1989) Efficient production of haploid wheat (Triticum aestivum) through crosses between Japanese wheat and maize (Zea mays). Plant Cell Rep 8:263–266
Inagaki MN (1990) Wheat haploids through the bulbosum technique. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry. Springer, Berlin, pp 448–459
Inagaki MN, Nagamine T, Mujeeb-Kazi A (1997) Use of pollen storage and detached-tiller culture in wheat polyhaploid production through wide crosses. Cereal Res Commun 25(1):7–13
Murashige T, Skoog S (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
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Santra, M., Wang, H., Seifert, S., Haley, S. (2017). Doubled Haploid Laboratory Protocol for Wheat Using Wheat–Maize Wide Hybridization. In: Bhalla, P., Singh, M. (eds) Wheat Biotechnology. Methods in Molecular Biology, vol 1679. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7337-8_14
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DOI: https://doi.org/10.1007/978-1-4939-7337-8_14
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7337-8
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