Abstract
Internal oxygen concentrations vary in different tissues depending on tissue size, developmental stage, and their location. Respiratory rate of tissue also determines internal oxygen levels. For studying various signaling pathways it is essential to establish a correlation between respiration and internal oxygen. Seed germination is associated with increase in respiration which can dictate the internal oxygen and subsequent production of reactive oxygen species. Using optic oxygen microsensor we made an attempt to measure respiratory rate and internal oxygen. We found that microsensor is able to sense internal oxygen and it is also possible to measure oxygen levels in a close vial that contains seeds. Step-by-step protocol is described here along with illustration.
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References
Armstrong W, Brändle R, Jackson MB (1994) Mechanisms of flood tolerance in plants. Acta Bot Neerl 43:307–358
Crawford R, Brändle R (1996) Oxygen deprivation stress in a changing environment. J Exp Bot 47:145–159
Drew MC (1997) Oxygen deficiency and root metabolism: injury and acclimation under hypoxia and anoxia. Annu Rev Plant Physiol Plant Mol Biol 48:223–250
Borisjuk L, Macherel D, Benamar A, Wobus U, Rolletschek H (2007) Low oxygen sensing and balancing in plant seeds – a role for nitric oxide. New Phytol 176:813–823
Rolletschek H, Radchuk R, Klukas C, Schreiber F, Wobus U, Borisjuk L (2005) Evidence of a key role for photosynthetic oxygen release in oil storage in developing soybean seeds. New Phytol 167:777–786
Gupta KJ, Hebelstrup KH, Kruger NJ, Ratcliffe RG (2014) Nitric oxide is required for homeostasis of oxygen and reactive oxygen species in barley roots under aerobic conditions. Mol Plant 7:747–750
Gupta KJ, Stoimenova M, Kaiser WM (2005) In higher plants, only root mitochondria, but not leaf mitochondria reduce nitrite to NO, in vitro and in situ. J Exp Bot 56:2601–2609
Vergara R, Parada F, Rubio S, Pérez FJ (2012) Hypoxia induces H2O2 production and activates antioxidant defence system in grapevine buds through mediation of H2O2 and ethylene. J Exp Bot 63:4123–4131
Acknowledgements
This work was supported by NIPGR short term research fellowship to S.P., and IYBA to A.K. and K.J.G. from Department of Biotechnology.
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Pandey, S., Kumari, A., Bharadwaj, C., Gupta, K.J. (2017). Measurement of Respiration and Internal Oxygen in Germinating Cicer arietinum L. Seeds Using Optic Microsensor. In: Jagadis Gupta, K. (eds) Plant Respiration and Internal Oxygen. Methods in Molecular Biology, vol 1670. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7292-0_6
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DOI: https://doi.org/10.1007/978-1-4939-7292-0_6
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7291-3
Online ISBN: 978-1-4939-7292-0
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