Abstract
Fibrinogen is measured in plasma most commonly using the Clauss method, based on the comparison of thrombin clotting times of dilutions of plasma against a plasma standard. Thrombin time (TT) is a coagulation assay, which reflects the conversion of fibrinogen to fibrin after addition of thrombin reagent. Measurement of clottable fibrinogen and TT allows detecting inborn (congenital) and acquired qualitative and quantitative disorders of fibrinogen that can lead to thrombotic or bleeding events.
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References
Undas A (2014) Fibrin clot properties and their modulation in thrombotic disorders. Thromb Haemost 112:32–42
Mosesson MW (2005) Fibrinogen and fibrin structure and functions. J Thromb Haemost 3:1894–1904
Mackie IJ, Kitchen S, Machin SJ, Lowe GD, Haemostasis and Thrombosis Task Force of the British Committee for Standards in Haematology (2003) Guidelines on fibrinogen assays. Br J Haematol 121:396–404
Multifibren U (2008) Package insert. Siemens Healthcare Diagnostics Products GmbH, Marburg Germany. Edition May 2008
Clauss A (1957) Gerinnungsphysiologische Schnellmethode zu Bestimmung des Fibrinogens. Acta Haematol 17:237–245
Undas A (2016) How to assess fibrinogen levels and fibrin clot properties in clinical practice? Semin Thromb Hemost 42:381–388
Adcock DM, Bethel MA, Macy PA (2002) Coagulation handbook. Esoterix, Inc. Laboratory Services. pp 134–136
Chandler WL (2009) Initial evaluation of hemostasis: reagent and method selection. In: Kitchen S, Olson JD, Preston FE (eds) Quality in laboratory hemostasis and thrombosis. Wiley-Blackwell, Oxford, pp 63–71
Fickenscher K (1998) Thrombin time (TT) test. In: Lothar T (ed) Clinical laboratory diagnostics: use and assessment of clinical laboratory results. TH-Books Verlagsgesellschaft mbH, Frankfurt/Main, Germany, pp 604–605
Flanders MM, Crist R, Rodgers GM (2003) Comparison of five thrombin time reagents. Clin Chem 49:169–712
Ignjatovic V (2013) Thrombin clotting time. Methods Mol Biol 992:131–138
Siemens Healthcare Diagnostics Products GmbH (2009) Fibrinogen Calibrator Kit package insert. Siemens Healthcare Diagnostics Products GmbH, Marburg, Germany. Edition October 2009
Siemens Healthcare Diagnostics Products GmbH (2015) Control Plasma N package insert. Siemens Healthcare Diagnostics Products GmbH, Marburg, Germany. Edition July 2015
Siemens Healthcare Diagnostics Products GmbH (2007) Control Plasma P package insert. Siemens Healthcare Diagnostics Products GmbH, Marburg, Germany. Edition November 2007
Siemens Healthcare Diagnostics Products GmbH (2010) BC Thrombin Reagent package insert. Siemens Healthcare Diagnostics Products GmbH, Marburg, Germany. Edition March 2010
Monagle P, Barnes C, Ignjatovic V, Furmedge J, Newall F, Chan A, De Rosa L, Hamilton S, Ragg P, Robinson S, Auldist A, Crock C, Roy N, Rowlands S (2006) Developmental haemostasis. Impact for clinical haemostasis laboratories. Thromb Haemost 95:362–372
Cunningham MT, Brandt JT, Laposata M, Olson JD (2002) Laboratory diagnosis of dysfibrinogenemia. Arch Pathol Lab Med 126:499–505
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Undas, A. (2017). Determination of Fibrinogen and Thrombin Time (TT). In: Favaloro, E., Lippi, G. (eds) Hemostasis and Thrombosis. Methods in Molecular Biology, vol 1646. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7196-1_8
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DOI: https://doi.org/10.1007/978-1-4939-7196-1_8
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