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Detection of Naturally Occurring Human Antibodies Against Gangliosides by ELISA

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Natural Antibodies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1643))

Abstract

Gangliosides are sialic acid-containing glycolipids that have been considered attractive targets for cancer immunotherapy, based on the qualitative and quantitative changes they suffer during malignant transformation and due to their importance for tumor biology. Natural antibodies against gangliosides have been detected not only in cancer patients but also in healthy donors. The presence of these antibodies can be used as diagnostic or prognostic factor. However, these responses are difficult to detect because anti-ganglioside antibodies are usually of IgM isotype and low affinity. Enzyme Linked Immunosorbent Assay (ELISA) is an immunoassay based on the specific binding of antibodies to antigens bound to a solid phase. These antigens can be glycolipids like gangliosides. An enzyme linked to the last reactant allows the detection of specific binding through the development of color after the addition of a suitable substrate. ELISA combines the specificity of antibodies with the sensitivity of enzyme reactions. The ELISA method described herein can be used to detect antibody responses against gangliosides not only related to cancer but also to autoimmune diseases and infections, both in healthy donors, and patients, untreated or receiving specific immunotherapy.

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Author information

Authors and Affiliations

  1. Center for Molecular Immunology, 11600, Havana, Cuba

    Ana María Hernández & Nely Rodríguez-Zhurbenko

Authors
  1. Ana María Hernández
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  2. Nely Rodríguez-Zhurbenko
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Correspondence to Ana María Hernández .

Editor information

Editors and Affiliations

  1. Centre de Recherche des Cordeliers, INSERM, PARIS, Paris, France

    Srinivas V. Kaveri

  2. Centre de Recherche des Cordeliers, INSERM, PARIS, Paris, France

    Jagadeesh Bayry

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Hernández, A.M., Rodríguez-Zhurbenko, N. (2017). Detection of Naturally Occurring Human Antibodies Against Gangliosides by ELISA. In: Kaveri, S., Bayry, J. (eds) Natural Antibodies. Methods in Molecular Biology, vol 1643. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7180-0_14

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  • DOI: https://doi.org/10.1007/978-1-4939-7180-0_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7179-4

  • Online ISBN: 978-1-4939-7180-0

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