Abstract
Detection of food-borne pathogens is traditionally carried out by plating out techniques in selective or differential media using Petri agar dishes or other culture-dependent methods, usually designed for each pathogen to be detected. These classical methods are time and personnel consuming and also may last for up to 5 days in the case of final confirmation of some specific pathogens.
Here we describe a method for fast multiplex detection of nine food-borne pathogens (all species usually required under most countrylegislations) by means of a single multiplex PCR reaction coupled to a capillary electrophoresis detection, in just 2–2.5 h and with a minimum cost of around 2 € per sample and nine pathogens. This method saves consumables and personnel time and allows a faster detection of any possible contaminated food batches at industrial level, therefore helping to prevent future food-borne outbreaks at clinical level.
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Acknowledgments
Authors wish to thank Government of Principality of Asturias-FICYT for financial support (Grants PEST08-17, IE09-257C1 and IE09-257C2).
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Villamizar-Rodríguez, G., Lombó, F. (2017). Multiplex Detection of Food-Borne Pathogens. In: Domingues, L. (eds) PCR. Methods in Molecular Biology, vol 1620. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-7060-5_10
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DOI: https://doi.org/10.1007/978-1-4939-7060-5_10
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Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4939-7059-9
Online ISBN: 978-1-4939-7060-5
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