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Real-Time Dual MRI for Predicting and Subsequent Validation of Intra-Arterial Stem Cell Delivery to the Central Nervous System

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Stem Cell Technologies in Neuroscience

Part of the book series: Neuromethods ((NM,volume 126))

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Abstract

Stem cell therapy for neurological disorders reached a pivotal point when the efficacy of several cell types was demonstrated in small-animal models. Translation of stem cell therapy is contingent upon overcoming the challenge of effective cell delivery to the human brain, which has a volume of ~1000 times larger than that of the mouse. Intra-arterial (IA) injection can achieve a broad, global, but if needed also spatially targeted biodistribution; however, its utility has been limited by unpredictable cell destination and homing as dictated by the vascular territory, as well as by safety concerns.

We show here that high-speed MRI can be used to visualize the intravascular distribution of a superparamagnetic iron oxide contrast agent and can thus be used to accurately predict the distribution of IA administered stem cells. Moreover, high-speed MRI enables the real-time visualization of cell homing, providing the opportunity for immediate intervention in the case of undesired biodistribution.

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Acknowledgments

We thank Mary McAllister for editorial assistance, and Lydia Gregg for preparing Figs. 1 and 4b.

Author information

Authors and Affiliations

  1. Division of MR Research, Russell H. Morgan Department of Radiology and Radiological Science, Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, The Johns Hopkins University, Baltimore, MD, USA

    Piotr Walczak & Miroslaw Janowski

  2. Department of Neurology and Neurosurgery, Faculty of Medical Sciences, University of Warmia and Mazury, Olsztyn, Poland

    Piotr Walczak

  3. NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

    Miroslaw Janowski

Authors
  1. Piotr Walczak
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  2. Miroslaw Janowski
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Corresponding author

Correspondence to Miroslaw Janowski .

Editor information

Editors and Affiliations

  1. Department of Pediatric Surgery Children’s Program in Regenerative Medicine, University of Texas Health Science Center at Houston McGovern Medical School, Houston, Texas, USA

    Amit K. Srivastava

  2. Center for Stem Cells and Regenerative Medicine, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, California, USA

    Evan Y. Snyder

  3. Departments of PM&R and Neurosurgery Harvard Medical School, Spaulding Rehabilitation Network and Brigham & Women’s Hospital, Boston, Massachusetts, USA

    Yang D. Teng

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Walczak, P., Janowski, M. (2017). Real-Time Dual MRI for Predicting and Subsequent Validation of Intra-Arterial Stem Cell Delivery to the Central Nervous System. In: Srivastava, A., Snyder, E., Teng, Y. (eds) Stem Cell Technologies in Neuroscience. Neuromethods, vol 126. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7024-7_12

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  • DOI: https://doi.org/10.1007/978-1-4939-7024-7_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7022-3

  • Online ISBN: 978-1-4939-7024-7

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