Abstract
Proper timing of degradation of maternal protein reserves is important for early embryonic development. The major modification that triggers proteins to degradation is ubiquitination, mediated by ubiquitin-proteolytic system. We focus here on Skp 1-Cul 1-F-box complex (SCF-complex), E3 ubiquitin-ligase, a part of ubiquitin-proteolytic system, which transfer ubiquitin to the substrate protein. We describe in this chapter the methods for the characterization of the expression profile of mRNA and protein of invariant members of SCF-complex and for the definition of SCF-complex activity.
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Acknowledgments
We thank R. Sucha and A. Susor for their helpful comments and expert assistance during the experiments and M. Kopcikova, J. Kankova, J. Supolikova, and M. Rakocyova for their technical assistance. This work was supported by the Czech Science Foundation (GACR 13-24730P), Grant Agency of Charles University in Prague (GAUK 1280214) and RVO 67985904.
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Benesova, V., Kinterova, V., Kanka, J., Toralova, T. (2017). Potential Involvement of SCF-Complex in Zygotic Genome Activation During Early Bovine Embryo Development. In: Lee, K. (eds) Zygotic Genome Activation. Methods in Molecular Biology, vol 1605. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6988-3_17
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DOI: https://doi.org/10.1007/978-1-4939-6988-3_17
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