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Development and Characterization of an Infectious cDNA Clone of Equine Arteritis Virus

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Reverse Genetics of RNA Viruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1602))

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Abstract

Development and characterization of several infectious cDNA clones of equine arteritis virus (EAV) have been described in the literature. Here we describe the assembly of the full-length infectious cDNA clone of the virulent Bucyrus strain (VBS; ATCC VR-796) of EAV in a plasmid vector. This system allows generation of infectious in vitro-transcribed (IVT) RNA from the linearized plasmid that can be transfected or electroporated into mammalian cells to produce infectious recombinant progeny virus. This is an efficient reverse genetics system that allows easy manipulation of EAV genomes to study molecular biology of the virus and pathogenesis of equine viral arteritis.

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Acknowledgments

This work was supported by Agriculture and Food Research Initiative competitive grant no. 2013-68004-20360 from the United States Department of Agriculture National Institute of Food and Agriculture (USDA-NIFA).

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Authors and Affiliations

  1. Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, 1400 Nicholasville Road, Lexington, KY, 40546, USA

    Udeni B. R. Balasuriya

  2. Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, 50011, USA

    Jianqiang Zhang

Authors
  1. Udeni B. R. Balasuriya
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  2. Jianqiang Zhang
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Corresponding author

Correspondence to Udeni B. R. Balasuriya .

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Editors and Affiliations

  1. Department of Population Health, Poultry Diagnostic and Research Center, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA

    Daniel R. Perez

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Balasuriya, U.B.R., Zhang, J. (2017). Development and Characterization of an Infectious cDNA Clone of Equine Arteritis Virus. In: Perez, D. (eds) Reverse Genetics of RNA Viruses. Methods in Molecular Biology, vol 1602. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6964-7_2

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  • DOI: https://doi.org/10.1007/978-1-4939-6964-7_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6962-3

  • Online ISBN: 978-1-4939-6964-7

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