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T Cell Receptor and Co-Stimulatory Signals for Th9 Generation

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Th9 Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1585))

Abstract

In vitro polarization of naïve CD4+ T cells toward distinct T helper lineages is crucial for establishing the factors and features that determine the differentiation, stability, and effector function for each T helper subsets. In this regard, the recently defined Th9 subset has been reported with two essential cytokines requirement for their generation. Generating Th9 cells in vitro from naïve CD4+ T cells requires the combination of TGF-β and IL-4. However, the amount of IL-9 producing under these minimal conditions is often small. The intent of this chapter is to provide examples to increase the generation of IL-9 producing T cells in vitro by modulating TCR strength and co-stimulation through the TNF family member TL1A. We hope that these methods to efficiently differentiate naïve CD4+ T cells toward IL-9 producing cells will facilitate understanding the differentiation and function of Th9 cells and their pathogenesis in various inflammatory and autoimmune diseases.

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Authors and Affiliations

  1. Immunoregulation Section, Autoimmunity Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, 10 Center Drive, Building 10, Room 13C120, Bethesda, MD, 20892, USA

    Françoise Meylan

  2. Genetic Disease Research Branch, National Human Genome Research Institute, National Institutes of Health, 9000 Rockville Pike., Bldg. 49, Room 4C64, Bethesda, MD, 20892, USA

    Julio Gomez-Rodriguez

Authors
  1. Françoise Meylan
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  2. Julio Gomez-Rodriguez
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Correspondence to Françoise Meylan or Julio Gomez-Rodriguez .

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Editors and Affiliations

  1. School of Bio Science, Sir JC Bose Laboratory Complex, Indian Institute of Technology, Kharagpur, West Bengal, India

    Ritobrata Goswami

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Meylan, F., Gomez-Rodriguez, J. (2017). T Cell Receptor and Co-Stimulatory Signals for Th9 Generation. In: Goswami, R. (eds) Th9 Cells. Methods in Molecular Biology, vol 1585. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6877-0_5

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  • DOI: https://doi.org/10.1007/978-1-4939-6877-0_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6876-3

  • Online ISBN: 978-1-4939-6877-0

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