Abstract
In a perspective of a comparative virology approach, characterization of the bovine leukemia virus (BLV) model may be helpful to better understand infection by the related human T-lymphotropic virus type 1 (HTLV-1). In this paper, we first provide detailed protocols to inoculate cloned BLV proviruses into sheep or cattle. We also describe methods to quantify apoptosis ex vivo and cell turnover in vivo.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Rodríguez SM, Florins A, Gillet N, de Brogniez A, Sánchez-Alcaraz MT, Boxus M, Boulanger F, Gutiérrez G, Trono K, Alvarez I, Vagnoni L, Willems L (2011) Preventive and therapeutic strategies for bovine leukemia virus: lessons for HTLV. Viruses 3(7):1210–1248
Gillet N, Florins A, Boxus M, Burteau C, Nigro A, Vandermeers F, Balon H, Bouzar A-B, Defoiche J, Burny A, Reichert M, Kettmann R, Willems L (2007) Mechanisms of leukemogenesis induced by bovine leukemia virus: prospects for novel anti-retroviral therapies in human. Retrovirology 4:18
Gutiérrez G, Rodríguez SM, De Brogniez A, Gillet N, Golime R, Burny A, Jaworski JP, Alvarez I, Vagnoni L, Trono K, Willems L (2014) Vaccination against δ-retroviruses: the bovine leukemia virus paradigm. Viruses 6(6):2416–2427
Lyons AB (2000) Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution. J Immunol Methods 243:147–154
Ristevski B (2003) Tracking dendritic cells: use of an in situ method to label all blood leukocytes. Int Immunol 15:159–165
Asquith B, Debacq C, Florins A, Gillet N, Sanchez-Alcaraz T, Mosley A, Willems L (2006) Quantifying lymphocyte kinetics in vivo using carboxyfluorescein diacetate succinimidyl ester. Proc R Soc B Biol Sci 273:1165–1171
Willems L, Kettmann R, Dequiedt F, Portetelle D, Vonèche V, Cornil I, Kerkhofs P, Burny A, Mammerickx M (1993) In vivo infection of sheep by bovine leukemia virus mutants. J Virol 67:4078–4085
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media LLC
About this protocol
Cite this protocol
de Brogniez, A. et al. (2017). Quantification of Cell Turnover in the Bovine Leukemia Virus Model. In: Casoli, C. (eds) Human T-Lymphotropic Viruses. Methods in Molecular Biology, vol 1582. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6872-5_13
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6872-5_13
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6870-1
Online ISBN: 978-1-4939-6872-5
eBook Packages: Springer Protocols