Abstract
The enzyme-linked immunosorbent assay (ELISA) is a widely employed analytical test used to quantify a given molecule. It relies on the use of specific antibodies, linked to an enzyme, to target the desired molecule. The reaction between the enzyme and its substrate gives rise to the analytical signal that can be quantified. Thanks to their robustness and low cost, molecularly imprinted polymer nanoparticles (nanoMIPs) are a viable alternative to antibodies. Herein, we describe the synthesis of nanoMIPs imprinted for vancomycin and their subsequent application in an ELISA-like format for direct replacement of antibodies.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Poma A, Turner AP, Piletsky SA (2010) Advances in the manufacture of MIP nanoparticles. Trends Biotechnol 28(12):629–637 Epub 2010/10/01
Canfarotta F, Poma A, Guerreiro A, Piletsky S (2016) Solid-phase synthesis of molecularly imprinted nanoparticles. Nat Protoc 11(3):443–455
Breton F, Rouillon R, Piletska EV, Karim K, Guerreiro A, Chianella I et al (2007) Virtual imprinting as a tool to design efficient MIPs for photosynthesis-inhibiting herbicides. Biosens Bioelectron 22(9–10):1948–1954
Surugiu I, Danielsson B, Ye L, Mosbach K, Haupt K (2001) Chemiluminescence imaging ELISA using an imprinted polymer as the recognition element instead of an antibody. Anal Chem 73(3):487–491
Surugiu I, Ye L, Yilmaz E, Dzgoev A, Danielsson B, Mosbach K et al (2000) An enzyme-linked molecularly imprinted sorbent assay. Analyst 125(1):13–16
Yonamine Y, Hoshino Y, Shea KJ (2012) ELISA-mimic screen for synthetic polymer nanoparticles with high affinity to target proteins. Biomacromolecules 13(9):2952–2957
Piletsky SA, Piletska EV, Bossi A, Karim K, Lowe P, Turner APF (2001) Substitution of antibodies and receptors with molecularly imprinted polymers in enzyme-linked and fluorescent assays. Biosens Bioelectron 16(9–12):701–707
Zhao D, Qiao X, Xu Z, Xu R, Yan Z (2013) Development of a biomimetic enzyme-linked immunosorbent assay method based on a hydrophilic molecularly imprinted polymer film for determination of olaquindox in chick feed samples. J Immunoassay Immunochem 34(1):16–29
Piletska EV, Guerreiro AR, Whitcombe MJ, Piletsky SA (2009) Influence of the polymerization conditions on the performance of molecularly imprinted polymers. Macromolecules 42(14):4921–4928
Chianella I, Guerreiro A, Moczko E, Caygill JS, Piletska EV, De Vargas Sansalvador IMP et al (2013) Direct replacement of antibodies with molecularly imprinted polymer nanoparticles in ELISA—Development of a novel assay for vancomycin. Anal Chem 85(17):8462–8468
Cáceres C, Canfarotta F, Chianella I, Pereira E, Moczko E, Esen C et al (2016) Does size matter? Study of performance of pseudo-ELISAs based on molecularly imprinted polymer nanoparticles prepared for analytes of different sizes. Analyst 141(4):1405–1412
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media LLC
About this protocol
Cite this protocol
Canfarotta, F., Smolinska-Kempisty, K., Piletsky, S. (2017). Replacement of Antibodies in Pseudo-ELISAs: Molecularly Imprinted Nanoparticles for Vancomycin Detection. In: Tiller, T. (eds) Synthetic Antibodies. Methods in Molecular Biology, vol 1575. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6857-2_25
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6857-2_25
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6855-8
Online ISBN: 978-1-4939-6857-2
eBook Packages: Springer Protocols