Abstract
Heat-induced antigen retrieval (HIAR) is an essential technique for current immunohistochemistry. We describe the mechanisms of HIAR and its protocols for formalin-fixed, paraffin-embedded specimens and frozen sections, which are the most popular materials for immunohistochemistry using light microscopy. In addition, we describe the antigen retrieval method for highly masked epitopes and double immunostaining using the horseradish peroxidase (HRP)-labeled antibody method and immunofluorescence method in this chapter.
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Yamashita, S., Katsumata, O. (2017). Heat-Induced Antigen Retrieval in Immunohistochemistry: Mechanisms and Applications. In: Pellicciari, C., Biggiogera, M. (eds) Histochemistry of Single Molecules. Methods in Molecular Biology, vol 1560. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6788-9_10
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DOI: https://doi.org/10.1007/978-1-4939-6788-9_10
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-6788-9
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