Abstract
Aberrant liver function as a consequence of excessive fat accumulation as observed in non-alcoholic fatty liver disease (NAFLD) is responsible for a plethora of disorders leading to the incidence of cardiovascular diseases. In most cases, the cause of NAFLD is not completely understood but has been primarily linked to the occurrence of insulin resistance that often accompanies obesity, dyslipidemia, and Type 2 Diabetes. Hence, it is of paramount importance to elucidate the molecular mechanisms of lipid metabolism underlying the pathogenesis of NAFLD and its comorbidities. Recent evidence indicates the role of lipid turnover in the pathophysiology of NAFLD via autophagy, namely lipophagy. Moreover, certain features of the liver such as its regenerative capacity make it an autophagy-dependent organ. This paper describes several autophagy molecular imaging tools that can be employed to visualize hepatic lipid turnover to elucidate the importance of this signaling cascade in pathological conditions, especially NAFLD.
The original version of this chapter was revised. The erratum to this chapter is available at: DOI 10.1007/978-1-4939-6759-9_21
An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-4939-6759-9_21
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
He C, Klionsky DJ (2009) Regulation mechanisms and signaling pathways of autophagy. Annu Rev Genet 43(January):67–93
Cuervo AM, Wong E (2014) Chaperone-mediated autophagy: roles in disease and aging. Cell Res 24(1):92–104
Rajat S, Kaushik S, Wang Y et al (2009) Autophagy regulates lipid metabolism. Nature 458(7242):1131–1135
Rizzetto M, Canese MG, Aricò S et al (1977) Immunofluorescence detection of new antigen-antibody system (delta/anti-delta) associated to hepatitis B virus in liver and in serum of HBsAg carriers. Gut 18(12):997–1003
Kimura S, Noda T, Yoshimori T (2007) Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3. Autophagy 3(5):452–460
Sainte-Marie G (1962) A paraffin embedding technique for studies employing immunofluorescence. J Histochem Cytochem 10(3):250–256
Ong KT, Mashek MT, Davidson NO et al (2014) Hepatic ATGL mediates PPAR-α signaling and fatty acid channeling through an L-FABP independent mechanism. J Lipid Res 55(5):808–815
Khan SA, Sathyanarayan A, Mashek MT et al (2015) ATGL-catalyzed lipolysis regulates SIRT1 to control PGC-1α/PPAR-α signaling. Diabetes 64(2):418–426
Acknowledgments
This work was supported by grants from the National Institutes of Health (DK090364) and the Minnesota Obesity Center (NIH DK050456) to Douglas G. Mashek.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media LLC
About this protocol
Cite this protocol
Sathyanarayan, A. (2017). A Coupled Approach Utilizing Immunohistochemistry and Immunocytochemistry to Visualize Cellular Lipophagy. In: Kalyuzhny, A. (eds) Signal Transduction Immunohistochemistry. Methods in Molecular Biology, vol 1554. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6759-9_11
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6759-9_11
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6757-5
Online ISBN: 978-1-4939-6759-9
eBook Packages: Springer Protocols