Abstract
Chromosome banding is an essential technique used in chromosome karyotyping to identify normal and abnormal chromosomes for clinical and research purposes. Giemsa (G)-, reverse (R)-, and centromere (C)-banding are the most commonly dye-based chromosome-banding techniques. G-banding involves the staining of trypsin-treated chromosomes and R-banding involves denaturing in hot acidic saline followed by Giemsa staining. C-banding is specifically used for identifying heterochromatin by denaturing chromosomes in a saturated alkaline solution followed by Giemsa staining. Different banding techniques may be selected for the identification of chromosomes.
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References
Gonon-Demoulian R, Goldman JM, Nicolini FE (2014) History of chronic myeloid leukemia: a paradigm in the treatment of cancer. Bull Cancer 101(1):56–67
Wan TS (2014) Cancer cytogenetics: methodology revisited. Ann Lab Med 34(6):413–425
Sudoyo AW, Hardi F (2011) Cytogenetics in solid tumors: lessons from the Philadelphia Chromosome. Acta Med Indones 43(1):68–73
Bernheim A (2010) Cytogenomics of cancer from chromosome to sequence. Mol Oncol 4(4):309–322
Swansbury J (2003) Introduction. In: Swansbury J (ed) Cancer cytogenetics: methods & protocols. Humana, Clifton, NJ
Ushiki T, Hoshi O, Iwai K et al (2002) The structure of human metaphase chromosomes: its histological perspective and new horizons by atomic force microscopy. Arch Histol Cytol 65(5):377–390
Craig JM, Bickmore WA (1993) Chromosome bands-flavours to savour. BioEssays 15:349–354
Saitoh Y, LaemmLi UK (1994) Metaphase chromosome structure: bands arise from a differential folding path of the highly AT-rich scaffold. Cell 76(4):609–622
Francke U (1994) Digitized and differentially shaded human chromosome ideograms for genomic applications. Cytogenet Cell Genet 65(3):206–218
McGowan-Jordan J, Simons A, Schmid M (eds) (2016) ISCN 2016: An International System for Human Cytogenomic Nomenclature. S. Karger, Basel. [Reprint of Cytogenet and Genome Res 149(1–2)]
Simons A, Shaffer LG, Hastings RJ (2013) Cytogenetic nomenclature: changes in the ISCN 2013 compared to the 2009 edition. Cytogenet Genome Res 141(1):1–6
Yunis JJ (1981) Mid-prophase human chromosomes: the attainment of 2000 bands. Hum Genet 56:293–298
Imai HT (1991) Mutability of constitutive heterochromatin (C-bands) during eukaryotic chromosomal evolution and their cytological meaning. Jpn J Genet 66(5):635–661
Wijayanto H, Hirai Y, Kamanaka Y et al (2005) Patterns of C-heterochromatin and telomeric DNA in two representative groups of small apes, the genera Hylobates and Symphalangus. Chromosome Res 13(7):717–724
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Huang, H., Chen, J. (2017). Chromosome Bandings. In: Wan, T. (eds) Cancer Cytogenetics. Methods in Molecular Biology, vol 1541. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6703-2_6
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DOI: https://doi.org/10.1007/978-1-4939-6703-2_6
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