Abstract
Human pluripotent stem cells (hPSC) are investigated as a source of authentic human cardiac cells for drug discovery and toxicological tests. Cell-based assays performed using an automated fluorescence imaging platform and high-content analysis are valuable in characterizing hypertrophic states that may be induced in hPSC-derived cardiomyocytes upon exposure to cardiotoxic compounds. In high-purity populations of hPSC-derived cardiomyocytes loaded with cell tracer probes and other cell markers, detailed hypertrophic profiles can be assessed based on information captured at cellular and subcellular levels.
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Acknowledgements
This work was supported by the UK Medical Research Council core funding to the MRC-UCL University Unit (Ref. MC_EX_G0800785) (JKV), the European Union Seventh Framework Programme (FP7/2007-2013, grant no. PIRG08-GA-2010-276811) (JKV), and the Hungarian Scientific Research Fund (OTKA K-105555) (GF).
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Glossary
- ANF
-
Atrial natriuretic peptide
- BNP
-
B-type natriuretic peptide
- ESC
-
Embryonic stem cell
- GSK
-
Glycogen synthase kinase
- HCM
-
Hypertrophic cardiomyopathy
- hESC
-
Human embryonic stem cell
- hESC-CM
-
Human embryonic stem cell-derived cardiomyocyte
- hiPSC
-
Human induced pluripotent stem cell
- hiPSC-CM
-
Human induced pluripotent stem cell-derived cardiomyocyte
- hPSC
-
Human pluripotent stem cell
- hPSC-CM
-
Human pluripotent stem cell-derived cardiomyocyte
- MAPK
-
Mitogen activated protein kinase
- NFAT
-
Nuclear factor of activated T-cells
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Kriston-Vizi, J., Harding, S.E., Földes, G. (2017). Structural Toxicity: Hypertrophy Models of Human Pluripotent Stem Cell-Derived Cardiomyocytes. In: Clements, M., Roquemore, L. (eds) Stem Cell-Derived Models in Toxicology. Methods in Pharmacology and Toxicology. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6661-5_14
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DOI: https://doi.org/10.1007/978-1-4939-6661-5_14
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