Abstract
Generating chimeric organisms is an invaluable way to study cell-to-cell movement and non-cell-autonomous actions of molecules. Plant grafting is an ancient method of generating chimeric organisms and recently has been used to study the movement of hormones, proteins, and RNAs. Here, I describe a simple and efficient way to graft Arabidopsis thaliana at the seedling stage to generate plants with roots and shoots of different genotypes. Using this protocol, success rates of over 80 % with up to 80 grafts assembled per hour can be achieved.
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Acknowledgement
I thank Elliot Meyerowitz and Raymond Wightman for critical reading. This work was funded by a Clare College Junior Research Fellowship and through Gatsby Charitable Trust grants GAT3272/C and GAT3273-PR1.
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Melnyk, C.W. (2017). Grafting with Arabidopsis thaliana . In: Kleine-Vehn, J., Sauer, M. (eds) Plant Hormones. Methods in Molecular Biology, vol 1497. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6469-7_2
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DOI: https://doi.org/10.1007/978-1-4939-6469-7_2
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