Abstract
Pig induced pluripotent stem cells (piPSCs) offer a great opportunity and a number of advantages in the generation of transgenic animals. These immortalized cells can undergo multiple rounds of genetic modifications (e.g., gene knock-in, knockout) and selection leading to animals that have optimized traits of biomedical or agricultural interests. In this chapter we describe the production and characterization of piPSCs, microinjection of piPSCs into embryos, embryo transfer and production of chimeric animals based on successful protocols.
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Acknowledgement
This work was supported by the Georgia Research Alliance Endowment and the University of Georgia Research Foundation.
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West, F.D., Terlouw, S.L., Dobrinsky, J.R., Lu, Y., Jordan, E.T., Stice, S.L. (2015). Generation of Chimeras from Porcine Induced Pluripotent Stem Cells. In: Verma, P., Sumer, H. (eds) Cell Reprogramming. Methods in Molecular Biology, vol 1330. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2848-4_14
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DOI: https://doi.org/10.1007/978-1-4939-2848-4_14
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2847-7
Online ISBN: 978-1-4939-2848-4
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