Abstract
Many proteins bind to nucleic acids. For the first characterization of novel proteins, a fast and simple technique for testing their nucleic acid binding capabilities is desirable. Here we describe the use of a North-western and South-western blot protocol for the evaluation of the DNA and RNA binding abilities of a novel putative methyl transferase HSPC133 (METTL5).
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References
Horke S, Reumann K, Schulze C, Grosse F, Heise T (2004) The La motif and the RNA recognition motifs of human La autoantigen contribute individually to RNA recognition and subcellular localization. J Biol Chem 279:50302–50309
Castro A, Faura M, Agell N, Renau-Piqueras J, Bachs O (1996) The autoantigen La/SSB is a calmodulin-binding protein. Cell Calcium 20:493–500
Zhang QH, Ye M, Wu XY, Ren SX, Zhao M, Zhao CJ et al (2000) Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells. Genome Res 10:1546–1560
Laemmli UK (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227:680–685
Bartsch H, Bachmann M (2009) Sequential use of immunoblots for characterization of autoantibody specificities. Methods Mol Biol 536:293–298
Acknowledgment
Special thanks for excellent technical support to Martina Franke (Inst. Immunology, Carl Gustav Carus TU-Dresden, Germany).
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© 2015 Springer Science+Business Media New York
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Franke, C., Gräfe, D., Bartsch, H., Bachmann, M.P. (2015). Use of Nonradioactive Detection Method for North- and South-Western Blot. In: Kurien, B., Scofield, R. (eds) Detection of Blotted Proteins. Methods in Molecular Biology, vol 1314. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2718-0_8
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DOI: https://doi.org/10.1007/978-1-4939-2718-0_8
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2717-3
Online ISBN: 978-1-4939-2718-0
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