Abstract
Alcian blue staining has been widely used to visualize acidic mucins and mucopolysaccharides in supported molecular matrix electrophoresis (SMME) and on membrane transferred from electrophoresis gels. Mucins with low acidic glycan content, however, cannot be stained with Alcian blue, which is one of the major drawbacks of this staining method. On the other hand, periodic acid-Schiff staining can selectively visualize glycoproteins, including mucins, regardless of the acidic residue content; however, periodic acid-Schiff staining decomposes glycans. Here, we introduce succinylation-Alcian blue staining as an alternative staining method to visualize mucins, regardless of the acidic residue content, and without glycan decomposition.
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Acknowledgement
This work was supported by a Grant-in-Aid for Scientific Research (KAKENHI 23310154) from the Japan Society for the Promotion of Science (JSPS).
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Kameyama, A., Dong, W., Matsuno, Yk. (2015). Succinylation-Alcian Blue Staining of Mucins on Polyvinylidene Difluoride Membranes. In: Kurien, B., Scofield, R. (eds) Detection of Blotted Proteins. Methods in Molecular Biology, vol 1314. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2718-0_33
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DOI: https://doi.org/10.1007/978-1-4939-2718-0_33
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2717-3
Online ISBN: 978-1-4939-2718-0
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