Abstract
Numerous software packages now exist for quantifying root growth responses, most of which analyze a time resolved sequence of images ex post facto. However, few allow for the real-time analysis of growth responses. The system in routine use in our lab allows for real-time growth analysis and couples this to positional feedback to control the stimulus experienced by the responding root. This combination allows us to overcome one of the confounding variables in studies of root gravity response. Seedlings are grown on standard petri plates attached to a vertical rotating stage and imaged using infrared illumination. The angle of a particular region of the root is determined by image analysis, compared to the prescribed angle, and any corrections in positioning are made by controlling a stepper motor. The system allows for the long-term stimulation of a root at a constant angle and yields insights into the gravity perception and transduction machinery not possible with other approaches.
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References
van der Weele CM, Jiang HS, Palaniappan KK, Ivanov VB, Baskin TI (2003) A new algorithm for computational image analysis of deformable motion at high spatial and temporal resolution applied to root growth. Roughly uniform elongation in the meristem and also, after an abrupt acceleration, in the elongation zone. Plant Physiol 132:1138–1148
Basu P, Pal A, Lynch JP, Brown KM (2007) A novel image-analysis technique for kinematic study of growth and curvature. Plant Physiol 145:305–316
Roberts TJ, McKenna SJ, Du C-J, Wuyts N, Valentine TA, Bengough AG (2010) Estimating the motion of plant root cells from in vivo confocal laser scanning microscopy images. Mach Vis Appl 21:921–939
French AP, Wilson MH, Kenobi K, Dietrich D, Voß U, Ubeda-Tomás S, Pridmore TP, Wells DM (2012) Identifying biological landmarks using a novel cell measuring image analysis tool: Cell-o-Tape. Plant Methods 8:7
Miller ND, Parks BM, Spalding EP (2007) Computer-vision analysis of seedling responses to light and gravity. Plant J 52:374–381
French A, Ubeda-Tomas S, Holman TJ, Bennett MJ, Pridmore T (2009) High-throughput quantification of root growth using a novel image-analysis tool. Plant Physiol 150:1784–1795
Russino A, Ascrizzi A, Popova L, Tonazzini A, Mancuso S, Mazzolai B (2013) A novel tracking tool for the analysis of plant-root tip movements. Bioinspir Biomim 8:025004
Armengaud P, Zambaux K, Hills A, Sulpice R, Pattison RJ, Blatt MR, Amtmann A (2009) EZ-Rhizo: integrated software for the fast and accurate measurement of root system architecture. Plant J 57:945–956
Mairhofer S, Zappala S, Tracy SR, Sturrock C, Bennett M, Mooney SJ, Pridmore T (2012) RooTrak: automated recovery of three-dimensional plant root architecture in soil from X-ray microcomputed tomography images using visual tracking. Plant Physiol 158:561–569
Ristova D, Rosas U, Krouk G, Ruffel S, Birnbaum KD, Coruzzi GM (2013) RootScape: a landmark-based system for rapid screening of root architecture in Arabidopsis. Plant Physiol 161:1086–1096
Pound MP, French AP, Atkinson J, Wells DM, Bennett MJ, Pridmore TP (2013) RootNav: Navigating images of complex root architectures. Plant Physiol 162:1802–1814
Mullen JL, Wolverton C, Ishikawa H, Evans ML (2000) Kinetics of constant gravitropic stimulus responses in Arabidopsis roots using a feedback system. Plant Physiol 123:665–670
Wolverton C, Paya AM, Toska J (2011) Root cap angle and gravitropic response rate are uncoupled in the Arabidopsis pgm-1 mutant. Physiol Plant 141:373–382
Wolverton C, Mullen JL, Ishikawa H, Evans ML (2002) Root gravitropism in response to a signal originating outside of the cap. Planta 215:153–157
Kiss JZ, Mullen JL, Correll MJ, Hangarter RP (2003) Phytochromes A and B mediate red-light-induced positive phototropism in roots. Plant Physiol 131:1411–1417
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The author acknowledges helpful feedback on the manuscript from Jayne Ackerman, Dara Markus, and Katelyn Sageman.
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Wolverton, C. (2015). Quantification of Root Gravitropic Response Using a Constant Stimulus Feedback System. In: Blancaflor, E. (eds) Plant Gravitropism. Methods in Molecular Biology, vol 1309. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2697-8_3
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DOI: https://doi.org/10.1007/978-1-4939-2697-8_3
Publisher Name: Humana Press, New York, NY
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