Abstract
Thirty-five blood group systems, containing more than 300 antigens, are listed by the International Society of Blood Transfusion (ISBT). Most of these antigens result from a single-nucleotide polymorphism (SNP). Blood group typing is conventionally carried out by serology. However, this technique has certain limitations and cannot respond to the growing demand for blood products typed for a large number of antigens. Here we describe a blood group genotyping assay, from genomic DNA extraction from whole-blood samples to results. After DNA extraction, the on-chip test is based on the hybridization of targets beforehand amplified by multiplex polymerase chain reaction, followed by a revelation step allowing the simultaneous identification of up to 24 blood group antigens and leading to the determination of extended genotypes.
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Boccoz, S.A., Le Goff, G., Blum, L.J., Marquette, C.A. (2015). Microarrays in Blood Group Genotyping. In: Bugert, P. (eds) Molecular Typing of Blood Cell Antigens. Methods in Molecular Biology, vol 1310. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2690-9_9
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DOI: https://doi.org/10.1007/978-1-4939-2690-9_9
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2689-3
Online ISBN: 978-1-4939-2690-9
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