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Parallel Donor Genotyping for 46 Selected Blood Group and 4 Human Platelet Antigens Using High-Throughput MALDI-TOF Mass Spectrometry

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Molecular Typing of Blood Cell Antigens

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1310))

Abstract

Most blood group antigens are defined by single nucleotide polymorphisms (SNPs). Highly accurate MALDI-TOF MS has proven its potential in SNP genotyping and was therefore chosen for blood donor oriented genotyping with high-throughput capability, e.g., 380 samples per day. The Select Module covers a total of 36 SNPs in two single-tube reactions, representative of 46 blood group and 4 human platelet antigens. Using this tool, confirmatory blood group typing for RhD, RhCE, Kell, Kidd, Duffy, MN, Ss, and selected rare antigens is performed on a routine basis.

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Acknowledgements

Financial support for the development of the Select Module was granted by the Humanitarian Foundation of the Swiss Red Cross (SRC); the Blood Transfusion Service Zurich, SRC, Switzerland and the Swiss blood transfusion umbrella organization Blutspende SRK Schweiz, Bern, Switzerland. SM, NT, and BMF are employees of the Blood Transfusion Service Zurich, SRC, Switzerland, and have not disclosed any conflicts of interest. CG is employee of the Blood Transfusion Service Zurich, SRC, Switzerland, and acts as a consultant for Inno-Train GmbH, Kronberg im Taunus, Germany.

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Authors and Affiliations

  1. Department of Molecular Diagnostics and Research (MOC), Swiss Red Cross Blood Transfusion Service Zurich, Rütistrasse 19, 8952, Schlieren, Switzerland

    Stefan Meyer, Nadine Trost, Beat M. Frey & Christoph Gassner

Authors
  1. Stefan Meyer
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  2. Nadine Trost
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  3. Beat M. Frey
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  4. Christoph Gassner
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Corresponding author

Correspondence to Christoph Gassner .

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Editors and Affiliations

  1. Medical Faculty Mannheim, German Red Cross Blood Service Baden-Wurttemberg – Hessen, Institute of Transfusion Medicine and Immunology, Heidelberg University, Mannheim, Germany

    Peter Bugert

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Meyer, S., Trost, N., Frey, B.M., Gassner, C. (2015). Parallel Donor Genotyping for 46 Selected Blood Group and 4 Human Platelet Antigens Using High-Throughput MALDI-TOF Mass Spectrometry. In: Bugert, P. (eds) Molecular Typing of Blood Cell Antigens. Methods in Molecular Biology, vol 1310. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2690-9_5

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  • DOI: https://doi.org/10.1007/978-1-4939-2690-9_5

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2689-3

  • Online ISBN: 978-1-4939-2690-9

  • eBook Packages: Springer Protocols

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