Abstract
This chapter contains some advice for first validating a new genotyping method and then continuing to ensure that it performs well when adopted for routine use. It should be applicable to most of the genotyping methods described in this volume; however, because the range of techniques is so wide and these recommendations have been written with PCR amplification in mind, the reader may need to adapt some of the recommendations for non-PCR based tests.
It does not include any advice on how to handle unscreened human samples within the laboratory, and it assumes that the laboratory already has established good practice for DNA amplification and the avoidance of contamination.
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© 2015 Springer Science+Business Media New York
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Metcalfe, P. (2015). Validation of Genotyping Protocols for Diagnostic Use. In: Bugert, P. (eds) Molecular Typing of Blood Cell Antigens. Methods in Molecular Biology, vol 1310. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2690-9_1
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DOI: https://doi.org/10.1007/978-1-4939-2690-9_1
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2689-3
Online ISBN: 978-1-4939-2690-9
eBook Packages: Springer Protocols