Abstract
Respiratory measurement in plants is one of the commonly used techniques to assess metabolic activity and in vivo redox state of plant mitochondria. However, respiration rate monitoring of Arabidopsis (Arabidopsis thaliana) remains a challenge for researchers due to the small size of its organs. In this chapter we introduce adaptations to micro-respiratory technologies to study three tissues of special interest to plant biologists: leaf sections, root tips, and seeds in this model plant species. This assay opens up new possibilities to screen and study mutants and to identify differences in ecotypes or populations of plants.
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References
Amthor JS (1989) Respiration and crop productivity. Springer, New York, NY
Lancien M, Gadal P, Hodges M (2000) Enzyme redundancy and the importance of 2-oxoglutarate in higher plant ammonium assimilation. Plant Physiol 123:817–824
Raghavendra AS, Padmasree K (2003) Beneficial interactions of mitochondrial metabolism with photosynthetic carbon assimilation. Trends Plant Sci 8:546–553
Krömer S (1995) Respiration during photosynthesis. Annu Rev Plant Physiol Plant Mol Biol 46:45–70
Plaxton WC (1996) The organization and regulation of plant glycolysis. Annu Rev Plant Physiol Plant Mol Biol 47:185–214
Plaxton WC (2004) Plant response to stress: biochemical adaptations of to phosphate deficiency. In: Goodman R (ed) Encyclopedia of plant and crop science. CRC Press, New York, NY, pp 976–980
Roberto Millan-Almaraz J, Gerardo Guevara-Gonzalez R, de Jesus Romero-Troncoso R, Alfredo Osornio-Rios R, Torres-Pacheco I (2009) Advantages and disadvantages on photosynthesis measurement techniques: a review. Afr J Biotechnol 8:7340–7349
Hunt S (2003) Measurements of photosynthesis and respiration in plants. Physiol Plant 117:314–325
Meyer EH, Tomaz T, Carroll AJ, Estavillo G, Delannoy E, Tanz SK, Small ID, Pogson BJ, Millar AH (2009) Remodeled respiration in ndufs4 with low phosphorylation efficiency suppresses Arabidopsis germination and growth and alters control of metabolism at night. Plant Physiol 151:603–609
Tomaz T, Bagard M, Pracharoenwattana I, Linden P, Lee CP, Carroll AJ, Stroeher E, Smith SM, Gardestrom P, Millar AH (2010) Mitochondrial malate dehydrogenase lowers leaf respiration and alters photorespiration and plant growth in Arabidopsis. Plant Physiol 154:1143–1157
Wu M, Neilson A, Swift AL, Moran R, Tamagnine J, Parslow D, Armistead S, Lemire K, Orrell J, Teich J, Chomicz S, Ferrick DA (2007) Multiparameter metabolic analysis reveals a close link between attenuated mitochondrial bioenergetic function and enhanced glycolysis dependency in human tumor cells. Am J Physiol Cell Physiol 292:C125–C136
O’Riordan TC, Zhdanov AV, Ponomarev GV, Papkovsky DB (2007) Analysis of intracellular oxygen and metabolic responses of mammalian cells by time-resolved fluorometry. Anal Chem 79:9414–9419
Schuh RA, Clerc P, Hwang H, Mehrabian Z, Bittman K, Chen H, Polster BM (2011) Adaptation of microplate-based respirometry for hippocampal slices and analysis of respiratory capacity. J Neurosci Res 89:1979–1988
Choi SW, Gerencser AA, Nicholls DG (2009) Bioenergetic analysis of isolated cerebrocortical nerve terminals on a microgram scale: spare respiratory capacity and stochastic mitochondrial failure. J Neurochem 109:1179–1191
Dykens JA, Jamieson JD, Marroquin LD, Nadanaciva S, Xu JJ, Dunn MC, Smith AR, Will Y (2008) In vitro assessment of mitochondrial dysfunction and cytotoxicity of nefazodone, trazodone, and buspirone. Toxicol Sci 103:335–345
Rogers GW, Brand MD, Petrosyan S, Ashok D, Elorza AA, Ferrick DA, Murphy AN (2011) High throughput microplate respiratory measurements using minimal quantities of isolated mitochondria. PLoS One 6:e21746
Gerencser AA, Neilson A, Choi SW, Edman U, Yadava N, Oh RJ, Ferrick DA, Nicholls DG, Brand MD (2009) Quantitative microplate-based respirometry with correction for oxygen diffusion. Anal Chem 81:6868–6878
Sew YS, Stroeher E, Holzmann C, Huang S, Taylor N, Jordana X, Millar AH (2013) Multiplex micro-respiratory measurements of Arabidopsis tissues. New Phytol 200:922–932
Atkin OK, Cummins WR, Collier DE (1993) Light Induction of alternative pathway capacity in leaf slices of Belgium endive. Plant Cell Environ 16:231–235
Armstrong AF, Logan DC, Atkin OK (2006) On the developmental dependence of leaf respiration: responses to short- and long-term changes in growth temperature. Am J Bot 93:1633–1639
Acknowledgments
Development of these protocols was funded by the Australian Research Council through the ARC Centre of Excellence in Plant Energy Biology (CE140100008). Y.S.S. is supported by a PhD scholarship from Malaysian Agricultural Research and Development Institute (MARDI). A.H.M. is supported by an ARC Future Fellowship (FT110100242) and E.S. by an ARC Postdoctoral Fellowship (DP110104865).
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Sew, Y.S., Millar, A.H., Stroeher, E. (2015). Micro-Respiratory Measurements in Plants. In: Whelan, J., Murcha, M. (eds) Plant Mitochondria. Methods in Molecular Biology, vol 1305. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2639-8_13
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DOI: https://doi.org/10.1007/978-1-4939-2639-8_13
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